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Flashcards · 9700 · AS Topic 1
Cell structure (AS Level) flashcards
17 cards on AS Topic 1, subtopics 1.1 to 1.2. Pick a subtopic or study them all.
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1.1 The microscope in cell studies
- Magnification
- How many times larger the image is than the actual size of the object. Magnification = image size ÷ actual size. It has no unit (write ×8000).
- Resolution
- The ability to distinguish two points that are close together as separate. It is not "how clear the image is".
- Unit conversions for cell measurements
- 1 mm = 1000 µm 1 µm = 1000 nm 1 mm = 1 000 000 nm Convert both measurements to the same unit before dividing.
- A nucleus on a TEM micrograph at ×14 000 measures 84 mm. What is its actual size?
- 84 mm = 84 000 µm. Actual size = image size ÷ magnification = 84 000 ÷ 14 000 = 6 µm.
- How do you calibrate an eyepiece graticule?
- Put a stage micrometer (a scale of known length) on the stage and line up the two scales. Find where lines coincide exactly, far along. One eyepiece unit = micrometer length ÷ number of eyepiece units. Recalibrate after changing the objective.
- Why do electron microscopes have a much better resolution than light microscopes?
- Resolution is limited by wavelength. An electron beam has a much shorter wavelength than light, so points much closer together can be distinguished. Light microscope limit: about 200 nm.
- TEM vs SEM
- TEM: the beam passes through an ultra-thin section; 2-D image of internal structure; highest resolution. SEM: the beam scans the surface; 3-D image of the surface. Both: dead specimens in a vacuum, black-and-white images.
- Rules for a plan diagram compared with a high-power drawing
- Plan diagram (low power): outlines of tissue layers in correct proportions, no individual cells. High-power drawing: individual cells, only visible contents, two lines for each cell wall, no shading, ruled label lines.
1.2 Cells as the basic units of living organisms
- Organelles surrounded by two membranes (an envelope)
- Nucleus (nuclear envelope with pores), mitochondria and chloroplasts.
- Cell structures with a single membrane
- Cell surface membrane, RER, SER, Golgi body, lysosomes, vesicles and the tonoplast.
- Cell structures with no membrane
- Ribosomes, nucleolus, centrioles, microtubules and the cell wall.
- Functions of the RER, SER and Golgi body
- RER: transports proteins made on its ribosomes. SER: makes lipids, including steroids. Golgi body: modifies and packages proteins; makes lysosomes.
- Cilia vs microvilli
- Cilia: long, with a 9 + 2 arrangement of microtubules and a basal body; they beat to move fluid or mucus. Microvilli: short folds of the cell surface membrane with no microtubule core; they increase surface area for absorption.
- Ribosomes in prokaryotic and eukaryotic cells
- Prokaryotes: 70S only. Eukaryotes: 80S in the cytoplasm and on the RER; 70S inside mitochondria and chloroplasts.
- Structural features of a typical bacterium
- Unicellular, generally 1–5 µm across, peptidoglycan cell wall, circular DNA free in the cytoplasm, 70S ribosomes, no double-membrane organelles.
- Structure of a virus
- Non-cellular. A nucleic acid core (DNA or RNA) inside a protein capsid. Some have an outer envelope of phospholipids.
- Why is "a bacterium has no organelles" wrong?
- A bacterium has ribosomes. It lacks a nucleus and double-membrane organelles such as mitochondria.
Revise Cell structure further
- Study guideCambridge International AS & A Level Biology 9700: Cells as the basic units of living organisms – Study Guide
- Revision notesCambridge International AS & A Level Biology 9700: Cells as the basic units of living organisms – Revision Notes
- Practice questionsCambridge International AS & A Level Biology 9700: Cells as the basic units of living organisms – Practice Questions